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. 2025 Jul 24;21(11):4798–4815. doi: 10.7150/ijbs.112943

Figure 2.

Figure 2

SETD1B is highly expressed in liver cancer stem cells (LCSCs). (A) (B) IF staining of EpCAM and OCT4 in HCCLM3/HepG2 cells and LCSCs. (C) (D) Flow cytometry (FC) detection of stemness markers (CD4 and CD133) in HCCLM3/HepG2 cells and LCSCs. (E) Tumor formation capabilities of HCCLM3 cells and HCCLM3 LCSCs in nude mice. (F) Statistical analysis of tumor weight. (G) Statistical analysis of tumor volumes. (H) Gene expression of SETD1B in HCCLM3/HepG2 cells and LCSCs. (I) Protein expression of SETD1B in HCCLM3/HepG2 cells and LCSCs. (J) Quantitative image analysis of SETD1B expression. ** p < 0.01, *** p < 0.001.