Skip to main content
. 2025 Jul 28;21(11):4979–4996. doi: 10.7150/ijbs.111449

Figure 1.

Figure 1

The sensitivity of NB cell lines to 13-cis RA is correlated with the expression of PLK4. A. Neurite extension assessment by measuring neurite length to cell body diameter in neuroblastoma cells treated with 0.1% DMSO or 10 μM 13-cis RA for 5 days. B. Immunofluorescence staining showing β-III tubulin expression and neurite outgrowth in SH-SY5Y cells treated with DMSO or 13-cis RA. C. Western blotting analysis of PLK4, SYN, MYCN, PHOX2B and GAP43 expression in 5 NB cell lines. D. Quantification of relative protein expression was performed, and data represent mean ± SEM from 3-4 independent experiments. E. Relative expressions of PLK4 and differentiation-associated markers were measured by quantitative reverse transcription PCR (RT-PCR). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: no significance.