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. 2025 Jul 28;21(11):4979–4996. doi: 10.7150/ijbs.111449

Figure 2.

Figure 2

PLK4 influences the differentiation of NB cells and PLK4-knockdown sensitizes them to the effects of 13-cis RA. A. PLK4 and differentiation-associated markers expression levels were assessed using Western blotting in NB cell lines (KD1: PLK4-knockdown1, KD2: PLK4-knockdown2, OE: PLK4 overexpression). B. Quantification of PLK4 and differentiation-associated markers expression was performed, and data represent mean ± SEM from 3-4 independent experiments. C & D. Expression levels of PLK4 and differentiation-associated markers were evaluated using RT-PCR in NB cell lines. E. Immunofluorescence of β-III Tubulin in SK-N-SH cells showing neurite outgrowths (red arrows) seen following PLK4-knockdown. F. Western blotting analysis of PLK4 and differentiation-associated markers protein levels in NB cell lines following PLK4-knockdown (the term KD as used in the text and figure pertains to KD2). G. Quantification of relative protein expression in 2G. Data were presented as mean ± SEM. n = 3-4. H. Rescue experiments assessing whether RA-induced differentiation is reversed by PLK4 overexpression in SK-N-SH. I. Quantification of above protein expression in 2H. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: no significance.