Skip to main content
. 2025 Jul 28;21(11):4979–4996. doi: 10.7150/ijbs.111449

Figure 4.

Figure 4

PLK4 mediates cell differentiation by interacting with CXCR4. A. Heatmap showing differentially expressed genes in PLK4-knockdown cells compared with the control cells. n = 3. B. Relative mRNA levels were of top differentially expressed genes were detected using RT-PCR. C. As shown in the left panel, Western blotting detected the expression of PLK4, CXCR4 and differentiation-associated markers after SH-SY5Y being treated with CFI-400945 or AMD-3100. The right panel corresponds to the quantification of relative protein expression. Data were presented as mean ± SEM. n = 3. D. Co-IP of endogenous as well as exogenous PLK4 interacts with CXCR4 in NB cells. E. PLK4-overexpression cells were treated with or without CCND1 siRNA, PLK4, CCND1 and differentiation-associated markers was examined. F. Quantification of relative protein expression in 3E. Data were presented as mean ± SEM. n = 3. Nuclear-cytoplasmic fractionation experiments (G) and Immunofluorescence (H-I) showed that PLK4 knockdown reduced the nuclear localization of cyclin D1. J. PLK4-overexpression cells were infected with LY294002 (5 μM, 48 h). The protein level of p-Akt and CCND1 were demonstrated by Western blotting. K. Quantification of relative protein expression in 3J (KD: PLK4-knockdown2, OE: PLK4 overexpression). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: no significance.