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. 2025 Jul 28;21(11):4979–4996. doi: 10.7150/ijbs.111449

Figure 6.

Figure 6

Inhibiting PLK4 can suppress the proliferation of NB cells and promote differentiation. A. IC50 values of CFI-400945 treatment in neuroblastoma cell lines for 72 h. IC50 was calculated in GraphPad Prism 8.0 and displayed respectively. B. Immunofluorescence of β-III Tubulin in SH-SY5Y cells treated with CFI-400945 or 0.1% DMSO for 5 days. C. Neurite extension assessment in neuroblastoma cells treated with CFI-400945 or 0.1% DMSO for 5 days. Treatment with CFI-400945 resulted in a significant increase in neurite outgrowth in NB cell lines, indicating differentiation of surviving cells. Data reported as mean ± SEM. D. Western blotting analysis of cells after treatment with CFI-400945 compared with the control cells. E. Quantification of relative protein expression in 6D. Data were presented as mean ± SEM. n = 3. F-G. Tumor development in SCID mice and tumor weights of tumors obtained from xenografts were assessed. H. Tumor volume in the all groups. I. The inhibition efficiency of PLK4, CXCR4 and the expression of differentiation-associated marker were examined by Western blotting. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: no significance.