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. 2025 Jul 28;21(11):4997–5014. doi: 10.7150/ijbs.113325

Figure 2.

Figure 2

NAT10 enhanced angiogenesis and tumor vessel abnormalization by upregulating VEGFA expression in GC cells. (A) Heatmap depicting significantly downregulated angiogenesis-related genes in NAT10-knockdown cells, as identified by RNA-sequencing. (B) Quantification of VEGFA mRNA levels in shNAT10 cells by qPCR. (C) Measurement of VEGFA protein levels in the culture supernatants of GC cells using enzyme-linked immunosorbent assay (ELISA). (D) Impact of Remodelin on the growth of human-derived gastric tumor organoids. Organoids were quantified, and their sizes were measured. Scale bar: 200 μm. (E) Representative IF images and quantification of VEGFA+ cells among 3D organoids. Scale bar: 20 μm. (F, G) Whole-body fluorescence images (F) and growth volume curves (G) of xenograft GC nude mouse model. (H, L) Representative images of Hypoxyprobe-1-labeled areas in MGC803 tumors. Scale bar: 50 μm. (I, M) Blood vessels were stained with the pan-endothelial marker CD31, and the nuclei were stained with DAPI. Scale bar: 200 μm. IF images of αSMA (J, N) and Collagen IV (K, O) in MGC803 tumors. The pericyte coverage and BM coverage were measured using ImageJ software.