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. 2025 Jul 28;21(11):5116–5134. doi: 10.7150/ijbs.109637

Table 1.

Synopsis of the synergistic action of nanoparticle and siRNA for tumor regression via immune cell activation.

Nanoparticle type used in synergy with siRNA Targeted immune
Cell
In-vivo In-vitro Gene targeted by siRNA Comments Reference
PGLA-based Dendritic Cells Lymphoma mice STAT3;
Silencing
Ovalbumin-specific T-cell activity was observed when NP was administered to lymphoma mice. Growth of tumor restricted. 68
Dendritic Cells B16F10 STAT3;
Silencing
CD86 expression upregulated,
Increased release of TNF-α,
Proliferation of allogenic T-cells
123
Dendritic Cells CD8 OVA 1.3 T cells SOCS1 Increased levels of TNF-a, IL-6, IL-12, IL-2,
More immunotherapeutic effects
68
PEI-based Dendritic Cells Lymphoma mice B16 melanoma STAT3;
Silencing
Dendritic cells are enabled to perform CTL activity,
Reduced growth of tumor in B16 mice.
122
Dendritic Cells Ovarian cancer mice HEK293 cells PD-L1;
Silencing
Induction of TLR5 and TLR7,
Restoration of the function of Dendritic cells in ovarian cancer mice.
59
Dendritic Cells Ovarian cancer mice miR-155 Increased miR-155 activity,
Anti-inflammatory mediators silenced,
Restoration of CTL activity of Dendritic cells in ovarian cancer mice.
124
Lipid-based Dendritic Cells Hep3b cell line PD-L1
PD-L2;
Knockdown
Knockdown of PD-L expression 120
Monocytes Lymphoma graft CCR2;
Silencing
No deposition of CCR2 in sites of inflammation,
Decrease in tumor volume
119
Lipid Envelope-based Dendritic Cells Cell line from Female C57BL/6 (H-2b) mice A20;
Silencing
LPS stimulation,
Increase in immunostimulatory cytokines
121
Stearylatedoctaargininelipid-based Dendritic Cells HeLa cells and E.G.,7-OVA cells SOCS1;
Silencing
Increased STAT1 phosphorylation,
Increase in immunostimulatory cytokines
111
Gold nanoparticles Macrophages Lung cancer mice Mouse BALB/c macrophage J774.2 cell line VEGF;
Silencing
Decrease in TAMs in lung tumor tissues,
Decrease in tumor size,
Enhancement of survival in mice
107
Mannosylated Polymeric Micelles Macrophages Breast cancer cell line CD206;
Knockdown
Improved delivery of the siRNAs,
90% knockdown
114