Figure 2.

Excessive iron deposition and ROS exhaustion in KD adipocytes. (A) Metascape-based analysis of the ferroptosis resistance-related genes, which are enriched in KD adipocytes. (B) Violin graphs show iron, ROS, and lipid metabolism gene expression in KN and KD skin-derived adipocyte cells. (C-C1) Immunofluorescence test and cell enumeration of PLIN1⁺GPX4⁺ adipocytes in keloid skin specimens. (D-D1) Immunofluorescence test and cell quantification of PLIN1⁺FTH1⁺ adipocytes in keloid skin specimens. (E-E1) Perl's staining and estimation of iron concentrations in dermal tissues. (F) Detection of intracellular iron levels in primary sorted adipocytes from KD and KN tissues. (G) Relative MDA levels by IHC assay. (H-I) qPCR and western blot examination of KN and KD primary fibroblast iron and ROS metabolism-related protein levels. The scale bar is 200 μm in C-E and 500-50 μm in G. N=6 per group. Data in B, C1, D1, E1, F, and G are derived from the Student's t-test. The data in H and I are analyzed with the Student's t-test and adjusted using the Benjamin-Hochberg procedure. Data are represented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.