Table 1.
Basal cellular parameters obtained for the cell culture experiments performed to obtain the data that are presented in the figures of this Article
| Initial ATP content | ATP content after 60 min BAM15 | Protein content | Initial LDH activity | n | |
|---|---|---|---|---|---|
| (nmol/mg) | (nmol/mg) | (µg/well) | nmol/(min x well) | ||
| Figure 1 | 32.8 ± 2.1 | 8.2 ± 1.8 | 119 ± 8 | 111 ± 5 | 3 |
| Figure 2 | 32.5 ± 1.2 | 8.0 ± 2.1 | 133 ± 14 | 115 ± 15 | 3 |
| Figure 3a | 29.6 ± 2.1 | 11.0 ± 2.8 | 135 ± 36 | 122 ± 35 | 3 |
| Figure 3b | 34.9 ± 4.0 | 6.1 ± 1.0 | 127 ± 2 | 101 ± 9 | 3 |
| Figure 4 | 29.8 ± 2.5 | 8.7 ± 1.7 | 146 ± 20 | 144 ± 53 | 3 |
| Figure 5 | 32.2 ± 1.7 | 7.5 ± 1.7 | 128 ± 13 | 112 ± 31 | 6 |
| Figure 6 | 32.2 ± 1.2 | 6.7 ± 3.2 | 147 ± 25 | 105 ± 20 | 3 |
| Figure 7 | 28.2 ± 1.0 | 5.8 ± 1.3 | 140 ± 14 | 105 ± 22 | 3 |
| Figure 8 | 29.9 ± 3.4 | 7.8 ± 2.2 | 152 ± 24 | 118 ± 19 | 3 |
| Average | 31.3 ± 3.0 | 7.8 ± 2.4 | 133 ± 20 | 115 ± 29 | 24 |
Shown are the initial specific contents of ATP, the protein contents, the total cellular LDH activities as well as the ATP contents determined after a 60 min preincubation with BAM15 to lower ATP content before the main incubation for ATP restoration was performed. In addition, the number of independent experiments (n) that were performed on independently prepared cultures is given. The given average values are derived from a total of 24 independent experiments performed on 21 independent cultures. This number is lower than the sum of the experiments used to obtain the data shown in the figures of this article, as data derived from different experiments that had been performed on the same culture on a given day were considered only once for the calculation of the average values