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. 2025 Aug 23;272(9):590. doi: 10.1007/s00415-025-13311-w

Table 2.

Functional exercise training studies in persons with multiple sclerosis

Design Sample Size Age [yrs] EDSS MS type Frequency Intensity Time Type Outcomes Between-group
Abbaspoor et al. 2020 RCT INT: 10 33.50 ± 6.37 3.06 ± 1.20 RRMS

3x/week

8 weeks

RPE

10–16

55–70% HRmax

5 exercises;

1–2 sets × 8–14 reps; 60–120 s rest

15–20 min

WB resistance

 BW; elastic bands; TRX® suspension 

Rhythmic AT

Serum markers (BDNF, IGF-1); walking speed (10MWT); walking endurance (2MWT); quadriceps strength (dynamometry); handgrip/finger strength (dynamometry)

INT: IGF-1 ↑;

10MWT ↑;

Handgrip strength (R) ↑;

BDNF ↔; 2MWT ↔; Handgrip strength (L) ↔; Finger strength (R/L) ↔ 

CON: 10 36.75 ± 6.80 3.00 ± 1.10 Habitual lifestyle
Akbar et al. 2020 CT INT1: 5 45.6 ± 12.8 N/A

RRMS

SPMS

PPMS

3x/week

16 weeks

N/A 10 exercises; 1–3 sets × 10–15 reps

WB resistance

BW; elastic bands; weighted vests       

Stretching

Fatigue (Modified Fatigue Impact Scale (total, physical, cognitive, psychosocial); physical activity (Godin Leisure-Time Exercise Questionnaire); grip strength (dynamometry); brain connectivity (resting-state functional MRI) INT1 vs. INT2: Functional connectivity caudate and inferior parietal region (L), inferior frontal region (R/L), middle frontal region (L), insula (R) ↕; modified Fatigue Impact Scale (total, physical, cognitive, psychosocial) ↔; Godin Leisure-Time Exercise Questionnaire ↔; grip strength
INT2: 5 53.0 ± 10.7 INT2: Stretching
Ayán Pérez et al. 2007 UCT INT: 24 (36)1 44.4 ± 9.5 1.5 ± N/A SPMS

3x/week

6 weeks

Self-chosen

(2–3 reps less than subjective max)

12 exercises; 1–2 sets x individual number of reps; ~ 2 min medicine ball throws;

60 min

Core strength and WB mobility

 BW; Calisthenics; medicine balls

Walking speed (9 m zig-zag run); mobility of arms and time–space orientation (clapping test, dynamic flexibility test); explosiveness of arms (overhead medicine ball throw); explosiveness of legs (vertical jump); trunk strength (abdominal test, back muscle test) leg lifts, Kraus-Weber test); Balance (flamingo balance test) -
Bilek et al. 2022 RCT INT: 30 28.29 ± 6.57 1.71 ± 1.13 RRMS

3x/week

8 weeks

N/A

70% V̇O2peak

60–70 min;

10 exercises;

1–3 sets × 10–15 reps; rest N/A

30 min

WB resistance

 Elastic bands;

weighted vests

AT (bicycle)

Cardioresp. fitness (V̇O2 peak); serum markers (Contactin-1, Contaction-2); Cognition (PASAT-3) INT vs. CON: V̇O2 peak ↑; Contactin-1 ↑; Contaction-2 ↑; PASAT-3 ↑
CON: 30 31.36 ± 8.07 2.00 ± 1.14 Habitual lifestyle
Cakt et al. 2010 RCT INT1: 10 43.0 ± 10.2 N/A

RRMS

SPMS

2x/week

8 weeks

N/A

15–25 min LB plyometrics + balance; 2 exercises LB plyometrics

15 sets × 2 min high-resistance cycling, 2 min low-resistance/rest

INT1: LB plyometrics

 INT 1: Balance exercise

  Walking, standing, balance board

Mobility (TUG); dynamic balance (Dynamic Gait Index); static balance (Functional Reach); fear of falling (Falls Efficacy Scale); walking speed (10MWT); fatigue (Fatigue Severity Scale); depression (Beck Depression Inventory); HRQoL (Short Form-36); total maximum workload; exercise duration

INT2 vs. INT 1 vs. CON: TUG ↑; Dynamic Gait Index ↑; Functional Reach ↑; Falls Efficacy Scale ↑; Fatigue Severity Scale ↑; Beck Depression Inventory ↑; total maximum workload ↑; exercise duration ↑; 10MWT ↔; SF-36 ↔ 

INT2 vs. CON: 10MWT ↑

INT2: 14 36.4 ± 10.5 40% total maximum workload

INT 2: Additional High-resistance cycling

 Bicycle ergometer

CON: 9 35.5 ± 10.9  Habitual lifestyle
Coote et al. 2015 RCT INT1: 10 51.8 ± 12.1 N/A

RRMS

SPMS

PPMS

BMS

2x/week

6 weeks;

3x/week

6 weeks

Adj. until muscular failure 6 exercises; 1–3 sets × 12 reps, 2–3 min rest

INT1: LB resistance

 BW, wooden box, free weights (hand, ankle, backpack)

Knee extension strength (dynamometry); hip extension strength (dynamometry); lower extremity strength (maximum rep Sit To Stand Test); lower-limb spasticity (VAS); mobility (TUG); self-reported walking impairment (MS Walking Scale–12); balance (Berg Balance Scale); impact of MS (MS Impact Scale-29); fatigue (Modified Fatigue Impact Scale) INT1 vs. INT2: Modified Fatigue Impact Scale (total) ↓; hip/knee extension strength ↔; Sit To Stand ↔; spasticity VAS ↔; TUG ↔; MS Walking Scale-12 ↔; MS Impact Scale (physiological/psychological score) ↔; Berg Balance Scale ↔ 
INT2: 15 51.8 ± 12.6 INT2: LB resistance combined with neuromuscular electric stimulation s.a
Correale et al. 2021 RCT INT: 14 45.4 ± 7.2 2.25 ± 0.8 RRMS

2x/week

12 weeks

Self-chosen

45–60 min;

3 sets × 8–12 reps; 60–90 s rest

25 min; 50–70% HRR

WB resistance

 Calisthenics; dumbbells; elastic bands

AT (bicycle/treadmill)

Strength quadriceps (MVIC, 1-RM leg extension); strength chest (1-RM chest press); strength back (1-RM seated row); fatigue (MFIS); depressive symptoms (BDI-II); HRQoL (MSQOL-54)

INT vs. CON: MFIS ↑; MSQOL-54 (mental composite) ↑;

MVIC ↔; 1-RM leg extension ↔; 1-RM chest press ↔; 1-RM seated row ↔; depressive symptoms ↔; MSQOL-54 (physical composite) ↔ 

CON: 13 48.3 ± 6.1  Habitual lifestyle
De Bolt and McCubbin 2004 RCT INT: 19 51.63 ± 6.71 3.97 ± 1.79

RRMS

PMS

BMS

3x/week

8 weeks

 ≥ 0.5% BW 25–30 min, 2–3 sets × 8–12 reps; self-chosen rests

LB resistance

 BW, weighted vests, ankle weights, step

Balance (anteroposterior sway, mediolateral sway, sway velocity); leg extensor power (maximal leg extension power); mobility (TUG) INT vs. CON: absolute/relative leg extensor power ↑; anteroposterior/mediolateral sway ↔; sway velocity ↔; TUG ↔ 
CON: 18 47.78 ± 10.47 3.50 ± 1.50  Habitual lifestyle
Frevel and Mäurer 2015 RCT INT1: 9 44.3 ± 8.1 3.8 ± 1.5 RRMS 6 (67) SPMS 3 (33)

2x/week

12 weeks

RPE 11–14 45 min; 5–8 exercises; 2–3 sets × 8–15 reps,

INT1: LB resistance BW, elastic bands, gym ball, mat

INT1: Balance    Standing, unstable surface

Balance (Berg Balance Scale; Dynamic Gait Index); knee extensor/flexor strength (MVIC); trunk extensor/flexor strength (MVIC); mobility (TUG); walking speed (2MWT 1 min/total); HRQoL (Hamburg Quality of Life Questionnaire in Multiple Sclerosis (HAQUAMS, total, all subscales); fatigue (Fatigue Severity Scale; Modified Fatigue Impact Scale) INT vs. INT2: MFIS cognitive subscale ↑; HAQUAMS upper limb ↑; Berg Balance Scale ↔; Dynamic Gait Index ↔; knee extensor/flexor MVIC ↔; trunk extensor/flexor MVIC ↔ 
INT2: 9 46.9 ± 7.6 3.8 ± 1.1 20–30 min INT2: Hippotherapy
Garrett et al. 2013 RCT INT1: 63# 51.7 ± 10 N/A

RRMS

SPMS

PPMS

BMS

1x/week

10 weeks

2x/week

10 weeks

Until muscular failure

65% HRmax,

RPE 11–14

60 min; 3 sets × 12 reps

30 min

30 min

INT1: WB resistance

 BW, free weights 

    INT1: AT          Mode of choice (walking/cycling/swimming/running)

HRQoL (Multiple Sclerosis Impact Scale-29 (physical/psychological)); fatigue (Modified Fatigue Impact Scale (total/physical/cognitive)); walking endurance (6MWT)

INT1 vs. CON: Multiple Sclerosis Impact

Scale-29 (physical/psychological) ↑; Modified Fatigue Impact Scale (total/physical) ↑; Modified Fatigue Impact Scale (cognitive) ↔;

6MWT ↑

INT2 vs. CON: Multiple Sclerosis Impact

Scale-29 (physical/psychological) ↑; Modified Fatigue Impact Scale (total/physical) ↑; Modified Fatigue Impact Scale (cognitive) ↔; 6MWT ↑

INT3 vs. CON: Multiple Sclerosis Impact

Scale-29 (psychological) ↑; Modified Fatigue Impact Scale (total/physical) ↑; Multiple Sclerosis Impact

Scale-29 (physical) ↔; Modified Fatigue Impact Scale (cognitive) ↔;

6MWT ↔ 

INT2: 67# 50.3 ± 10

1x/week

10 weeks

Not predefined Not predefined  INT2: Resistance training/AT/combined training Not predefined
INT3: 63# 49.6 ± 10

1x/week

10

Not predefined Not predefined INT3: Yoga Not predefined
CON: 49# 48.8 ± 11 Habitual lifestyle
Hosseini et al. 2018 RCT INT1: 9 32.9 ± 8.1 N/A N/A

3x/week

8 weeks

 ≥ 1% bodyweight 5 exercises; 3 sets × 10 reps, 30–60 s rest (betw. exercises)

INT1: LB resistance

 Free weights (fastened to the body)

Leg extensor strength (1-RM); leg strength (leg press); walking speed (10MWT); balance (postural sway balance index, two legs open/closed eyes, one leg open eyes) INT1 vs. INT2 vs. CON: Leg extensor 1-RM ↕ (INT1 ↑)
INT2: 9 31.3 ± 7.1 60–70 min; 1 set × 30–60 s, 30–60 s rest

INT2: Hatha Yoga

 Standing, sitting, lying

CON: 8 33.0 ± 9.7  Habitual lifestyle
Learmonth et al. 2012 RCT INT: 20 51.4 ± 8.06 6.14 ± 0.36 N/A

2x/week

12 weeks

Self-chosen

30–40 min;

8–12 exercises; N/A sets × 1 min;

 ≥ 60 s rest

WB resistance

 BW, dumbbells, chair

AT

 Walking/stepping/bike/Foot pedals

Balance

 Sitting/standing/walking

Walking speed (T25FW); body mass index; walking endurance (6MWT); balance (Berg Balance Scale); mobility (TUG); quadriceps strength (maximum isometric force leg extension, weaker leg); activity level (PhoneFITT); balance confidence (Activities Balance Confidence); fatigue (Fatigue Severity Scale); anxiety and depressive symptoms (HADS); HRQoL (LMSQOL); goal attainment (Goal Attainment Scale) T25FW ↔; PhoneFITT ↕; Activities Balance Confidence ↑ (0–12 wks); body mass index ↔; 6MWT ↔; Berg Balance Scale ↔; TUG ↔; maximum isometric force leg extension ↔; fatigue severity scale ↔; HADS ↔; LMSQOL ↔ 
CON: 12 51.8 ± 8.0 5.82 ± 0.51  Habitual lifestyle
Mardaniyan Ghahfarrokhi et al. 2022 RCT INT1: 15 39.87 ± 9.09 4.13 ± 0.97 RRMS

3x/week

8 weeks

CR-10:

2–6

120–190 min, 14 exercises, 1–2 sets × 10–12 reps

INT1: WB resistance

 Elastic bands

Static balance (wide/narrow, single-leg (R/L, open/closed eyes), semi-tandem, tandem open/closed eyes); dynamic balance (tandem walk test); mobility (TUG); walking endurance (6MWT); walking speed (10MWT, T25FWT); walking ability (6 Spot Step Test (dominant/non-dominant); lower extremity strength (5 Times Sit to Stand); hand grip strength (dynamometry) INT1 vs. INT2: Tandem walk ↓; Six Spot Step Test (non-dominant) ↓; 6MWT ↓; all other outcomes ↔ 
INT2: 15 37.50 ± 8.58 4.57 ± 1.30

CR-10:

2–6

90–120 min, 2 sets × 8 reps, 2 min rest

INT2: Neurofunctional exercise

 Balance (standing, walking), walking, WB resistance, pelvic control, core stability, BW, cones, Swiss ball

Moghadasi et al. 2020 CT INT: 19 37.62 ± 4.58 2.18 ± 0.85 RRMS:

3x/week

8 weeks

N/A 30 min; 8 exercises; 3 sets × 5–10 reps

WB Resistance

 TRX® suspension

Mobility (TUG); walking speed (10MWT); walking endurance (2MWT); lower extremity strength (5 Times Sit to Stand); joint position sense (knee proprioception absolute error); quadriceps/knee extensor strength (MVIC); knee flexor strength (MVIC) INT vs. CON: TUG ↑; 10MWT ↑; 2MWT ↑; 5 Times Sit to Stand ↑; knee proprioception absolute error non-dominant leg (60°) ↑;MVIC knee extensors dominant/non-dominant (20° and 70°) ↑; MVIC knee flexors dominant/non-dominant (20°) ↑; MVIC knee flexors dominant (70°) ↑; knee proprioception absolute error dominant leg (60°) ↔; proprioception absolute error dominant and non-dominant leg (30°) ↔; MVIC knee flexors non-dominant (70°) ↔ 
CON: 15 34.72 ± 5.01 2.59 ± 1.01  Habitual lifestyle
Motl et al. 2012 UCT INT: 13 51.5 ± 11.3 5.58 ± 0.79

RRMS

SPMS

PPMS

3x/week

8 weeks

RPE 13

RPE 13

RPE 13

15–60 min;

5–20 min; 5 exercises; sets/reps N/A

5–20 min

5–20 min

LB resistance

 Elastic bands

AT

 Ergometry (bicycle, rowing, elliptical)/treadmill

Balance

 Standing, walking

Walking speed (T25FW); self-reported walking impairment (MS Walking Scale-12); mobility (TUG); gait efficiency (Functional Gait Profile score, spatial and temporal gait measures)
Najafi et al., 2019 RCT INT: 30 38.39 ± 4.59 2.51 ± 1.22 RRMS

3x/week

8 weeks

N/A 45–60 min; 5 exercises; 3–4 sets × 10–15 s, 10–15 s rest

Core training

 BW

Balance

 Standing, walking (open/closed eyes)

Postural control (Center of Pressure sway area and path length, opened/closed eyes); balance (Berg Balance Scale); walking speed (T25FW); mobility (TUG) Center of Pressure sway area (opened/closed eyes) ↑; Center of Pressure path length (opened/closed eyes) ↑; Berg Balance Scale ↑; T25FW ↑; TUG ↑
CON: 30 36.36 ± 3.54 2.44 ± 0.77 N/A
Sabapathy et al. 2011 RCT

INT1:

11 (14)1

55 ± 7 N/A

RRMS

SPMS

PPMS

2x/week

8 weeks

CR10 3–5

8 exercises, 2–3 sets × 6–10 reps, ≥ 30–60 s rest

15–20 min

INT1: WB resistance and balance

 Elastic band, dumbbell (1–4 kg), parallel bar, Swiss ball, ankle weights (1–5 kg), foam mat, foam beam, wobble board                         INT1: Stretching

Grip strength (dynamometry); static balance (functional reach), dynamic balance (4 Step Square); mobility (TUG); walking endurance (6MWT); HRQoL (Multiple Sclerosis Impact Scale (physical/psychological); Short Form-36 (Physical and Mental Component Summary); fatigue (Modified Fatigue Impact Scale (physical/cognitive/psychosocial)); depressive symptoms (Becks Depression Inventory) INT1 vs. INT 2: all outcomes ↔ 

INT2:

5 (6)1

8 stations × 5 min, 2 min rest every 10 min

15–20 min

INT2: AT

 Arm crank, cycling, cross-trainer, step-ups, recumbent cycling, treadmill

INT2: Stretching

Sosnoff et al. 2014 RCT INT: 13 60.1 ± 6.3 5.5 (2.5)

RRMS

SPMS

PPMS

3x/week

12 weeks

N/A

45–60 min;

3 sets × 8–12 reps

LB and core resistance

 Elastic bands, BW

Balance

 Standing, walking

Fall risk (Physiological Profile Assessment total, subcomponents); balance (Berg Balance Scale); balance confidence (Activities-Specific Balance Confidence scale); walking speed (T25FW); walking endurance (6MWT); mobility (TUG); self-reported walking impairment (MS Walking Scale-12); self-reported fall frequency

INC vs. CON: Physiological Profile Assessment total ↑; postural sway subcomponent ↑; T25FW ↑; Activities-

Specific Balance Confidence scale ↑; other subcomponents (Melbourne Edge detection test, proprioception, strength, reaction time) ↔; 6MWT ↔; TUG ↔; MS Walking Scale-12 ↔; Berg Balance Scale ↔ 

CON: 14 60.1 ± 6.0

5.5 (3.5)

[Mdn (IQR)]

Habitual lifestyle
Zaenker et al. 2018 UCT

INT:

26 (30)1

44.6 ± 7.9 2.46 ± 1.52

RRMS

SPMS

PPMS

2x/week

1–4 week

3x/week

5–12 week

N/A

Intervals at 90–110% maximal tolerated power, rest at anaerobic threshold

moderate

4 exercises, 4–5 sets × 10–15 reps

s.a

17 min

35–45 min

LB resistance

 BW, resistance band, ankle weight

Additional LB resistance (5–12 weeks, every 2nd week)

AT

 HIIT: 5 intervals, 3 min rest

Additional AT (5–12 weeks, every 2nd week) Self-chosen

Cardiorespiratory fitness (VO2 peak, lactate at the end of the maximum aerobic test, HRpeak), physical capacity (maximal tolerated power), quadriceps strength (isokinetic peak torque), hamstring strength (isokinetic peak torque); HRQoL (MSQOL-54 total, subscales)

Intervention duration without warm-up/cool-down periods; #whole group prior drop-out; bold = in multicomponent interventions considered as “functional training”

 ↔  = no change, ↑ = significant improvement/between-group effect in favor of INT(1); ↓ = significant worsening/between-group in favor of INT2/CON; ↕ = unspecified significant difference, 1Data presented for n completing the program, initial sample size in brackets

AT aerobic training, BDNF brain-derived neurotrophic factor, BDI-II Beck depression inventory-II, BMS benign MS, CPET cardiopulmonary exercise test, CON control group, CT controlled trial, EDSS expanded disability status scale, f female, HRR heart rate reserve, HADS hospital anxiety and depression scale, HAQUAMS Hamburg quality of life questionnaire in multiple sclerosis, HRmax/HRpeak maximum/peak heart rate, HRQoL health-related quality of life, IGF-1 insulin like growth factor, INT intervention group, L left, LB lower body, m male, LMSQOL Leeds multiple sclerosis quality of life scale, MFIS modified fatigue impact scale, MRI magnetic resonance imaging, MSQOL-54 multiple sclerosis quality of life instrument-54 item, MVIC maximal voluntary isometric contraction, PASAT-3 paced auditory serial addition test with 3 s stimulus, PMS progressive MS, PPMS primary progressive MS, PSFS patient-specific functional scale, R right, RCT randomized controlled trial, RPE rate of perceived exertion, RRMS relapsing–remitting MS, RT resistance training, SF-36 Short Form-36, SPMS secondary progressive MS, TUG timed up and go, TSD time since diagnosis, T25FW timed 25-foot walk, UCT uncontrolled trial, VAS visual analog scale, V̇O2 max/V̇O2 peak maximum/peak oxygen consumption, WB whole body, 1-RM 1-repition maximum, 2MWT 2-min walk test, 6MWT 6-min walk test, 10MWT 10-m walk test