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. 2025 Jul 24;16(11):3403–3414. doi: 10.7150/jca.110597

Figure 3.

Figure 3

DUSP9 increased HNSCC cell proliferation and migration. (A) Detection of DUSP9 knockdown efficiency in CAL27 cells using siRNA by Western blot. The values between the two bands represent the DUSP9/β-actin ratio as measured by ImageJ. (B) Cell proliferation was determined using CCK-8 assay for CAL27 cells with DUSP9 knockdown. (C) Wound healing assay to examine the migration ability of CAL27 cells with DUSP9 knockdown. The healed areas were measured using ImageJ and calculated for percentage of cell migration. (D) Detection of DUSP9 overexpression efficiency in FaDu cells by Western blot. The values between the two bands represent the DUSP9/β-actin ratio as measured by ImageJ. (E) Cell proliferation was determined using CCK-8 assay for FaDu cells with DUSP9 overexpressed. (F) Wound healing assay to examine the migration ability of FaDu cells with DUSP9 overexpressed. The healed areas were measured using ImageJ and calculated for percentage of cell migration. Data were shown as mean ± SEM; p-value was determined by one-way ANOVA test (B, C) or Student's t-test (E, F); * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.