Table 1.
Properties of berovin mutants.
| Photoprotein | Yield of active protein, %* | Lspecific, RLU/mg, ×109 |
LCa-free, ×10− 8 |
BL λmax, nm |
FL*** λmax/shoulder λmax, nm (pH 8.5) |
|---|---|---|---|---|---|
| BAwt | 65 | 300 ± 0.22 (100%) | 0.4 | 490 | 398/492 |
| A106S | 60 | 300 ± 0.23 (100%) | 1.8 | 500 | 400/492 |
| A106T | 15 | 27.0 ± 0.08 (9%) | 25 | 505 | 425 |
| A106C | 60 | 180 ± 0.22 (60%) | 42 | 488 | 420/485 |
| A106V | 7 | 0.15 ± 0.001 (0.05%) | 125 | 480 | 430 |
| A106D | 5 | 0.09 ± 0.002 (0.03%) | 160 | ND** | 410 |
| A106N | 13 | 0.045 ± 0.001 (0.015%) | 5.5 | 485 | 418 |
| A106H | 7 | 0.09 ± 0.003 (0.03%) | 66 | ND | 425 |
| A106R | 17 | 0.06 ± 0.009 (0.02%) | 4.1 | 480 | 420 |
| A106Y | 30 | 0.075 ± 0.005 (0.025%) | 60 | 490 | 418/500 |
| A106W | 28 | 0.006 ± 0.0002 (0.002%) | 48 | 492 | 395/520 |
*The yield was estimated by averaging of at least two independent protein preparations.
**ND, not detected.
***The main fluorescence maximum is highlighted in bold.