Table 2.

Real-time PCR thermal cycling parameters.

Parameter	miR-361 and U6	ATAD1, HOMER1 and UBC
Initial Denaturation	94 °C for 10 min	95 °C for 15 min
Number of cycles	40	40
Denaturation	94 °C for 15 sec	95 °C for 30 sec
Annealing	64 °C for 30 sec	60 °C for 30 sec
Extension	64 °C for 30 sec	72 °C for 30 sec