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. 2025 Aug 23;15:31057. doi: 10.1038/s41598-025-10892-4

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© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 5 — Expression of Hedgehog signaling pathway-related proteins in colon tissues of mice in each group. Note: (A) Western blot bands of four proteins related to Hdgehog signaling pathway (Shh, Smo, Gli1, Ptch1) and intestinal tight junction proteins (Zo-1, Occuldin) in the colon tissues of each group of mice; (B–G) Western blot was used to detect the expressions of Hedgehog signaling pathways Shh, Smo, Gli1, Ptch1 and intestinal tight junction proteins Zo-1 and Occuldin in mice in each group. The data were expressed in Mean ± SD, n = 6; Compared with the model group, *P < 0.05, **P < 0.01, ***P < 0.001.