Table 2.
Pathway analysis depicting significantly altered metabolic pathways in Balanites aegyptiaca fruit extracts.
| Pathway | Total | Expected | Hits | Raw p | Holm adjust | FDR | Impact |
|---|---|---|---|---|---|---|---|
| Carbon fixation | 21 | 0.19626 | 4 | 2.62E−05 | 0.002433 | 0.002433 | 0.09578 |
| Galactose metabolism | 27 | 0.25234 | 4 | 7.43E−05 | 0.006833 | 0.003453 | 0.04805 |
| Glycine, serine and threonine metabolism | 33 | 0.30841 | 4 | 0.000168 | 0.015254 | 0.005196 | 0 |
| Citrate cycle (TCA cycle) | 20 | 0.18692 | 3 | 0.000675 | 0.060789 | 0.013987 | 0.15879 |
| Alanine, aspartate and glutamate metabolism | 22 | 0.20561 | 3 | 0.000902 | 0.08031 | 0.013987 | 0.1259 |
| Starch and sucrose metabolism | 22 | 0.20561 | 3 | 0.000902 | 0.08031 | 0.013987 | 0.40507 |
| Monobactam biosynthesis | 8 | 0.074766 | 2 | 0.002201 | 0.19145 | 0.029236 | 0 |
| Pyruvate metabolism | 23 | 0.21495 | 2 | 0.01835 | 1 | 0.21332 | 0.29205 |
| Glyoxylate and dicarboxylate metabolism | 29 | 0.27103 | 2 | 0.02852 | 1 | 0.2947 | 0.15112 |
| C5-Branched dibasic acid metabolism | 6 | 0.056075 | 1 | 0.05487 | 1 | 0.51029 | 0 |
| D-Amino acid metabolism | 7 | 0.065421 | 1 | 0.063739 | 1 | 0.5147 | 0 |
| Cysteine and methionine metabolism | 46 | 0.42991 | 2 | 0.066413 | 1 | 0.5147 | 0.01038 |
| Lysine biosynthesis | 9 | 0.084112 | 1 | 0.081245 | 1 | 0.58121 | 0 |
| Selenocompound metabolism | 13 | 0.1215 | 1 | 0.11535 | 1 | 0.67728 | 0 |
| Nicotinate and nicotinamide metabolism | 13 | 0.1215 | 1 | 0.11535 | 1 | 0.67728 | 0 |
| Butanoate metabolism | 17 | 0.15888 | 1 | 0.14827 | 1 | 0.67728 | 0 |
| Arginine biosynthesis | 18 | 0.16822 | 1 | 0.15632 | 1 | 0.67728 | 0 |
| Tyrosine metabolism | 18 | 0.16822 | 1 | 0.15632 | 1 | 0.67728 | 0 |
| beta-Alanine metabolism | 18 | 0.16822 | 1 | 0.15632 | 1 | 0.67728 | 0 |
| Fructose and mannose metabolism | 18 | 0.16822 | 1 | 0.15632 | 1 | 0.67728 | 0.06695 |
| Ascorbate and aldarate metabolism | 20 | 0.18692 | 1 | 0.17222 | 1 | 0.67728 | 0 |
| Cyanoamino acid metabolism | 21 | 0.19626 | 1 | 0.18006 | 1 | 0.67728 | 0 |
| One carbon pool by folate | 21 | 0.19626 | 1 | 0.18006 | 1 | 0.67728 | 0 |
| Thiamine metabolism | 22 | 0.20561 | 1 | 0.18783 | 1 | 0.67728 | 0 |
| Valine, leucine and isoleucine biosynthesis | 22 | 0.20561 | 1 | 0.18783 | 1 | 0.67728 | 0.10727 |
| Lipoic acid metabolism | 24 | 0.2243 | 1 | 0.20317 | 1 | 0.67728 | 0 |
| Tryptophan metabolism | 25 | 0.23364 | 1 | 0.21074 | 1 | 0.67728 | 0 |
| Pantothenate and CoA biosynthesis | 25 | 0.23364 | 1 | 0.21074 | 1 | 0.67728 | 0 |
| Glycolysis or Gluconeogenesis | 26 | 0.24299 | 1 | 0.21824 | 1 | 0.67728 | 0.11665 |
| Glutathione metabolism | 27 | 0.25234 | 1 | 0.22567 | 1 | 0.67728 | 0.01195 |
| Arginine and proline metabolism | 28 | 0.26168 | 1 | 0.23304 | 1 | 0.67728 | 0.03364 |
| Inositol phosphate metabolism | 28 | 0.26168 | 1 | 0.23304 | 1 | 0.67728 | 0.08958 |
| Terpenoid backbone biosynthesis | 31 | 0.28972 | 1 | 0.25476 | 1 | 0.71796 | 0 |
| Glycerophospholipid metabolism | 38 | 0.35514 | 1 | 0.30324 | 1 | 0.82945 | 0.02862 |
| Amino sugar and nucleotide sugar metabolism | 52 | 0.48598 | 1 | 0.39153 | 1 | 1 | 0 |
The table shows the detailed results from the pathway analysis generated with MetaboAnalyst 6.0. Since many pathways are being tested at the same time, the statistical p values from enrichment analysis are further adjusted for multiple testings. In particular, Total is the total number of compounds in the pathway; Hits is the matched number from the user uploaded data; Raw p is the original p value calculated from the enrichment analysis; Holm p is the p value adjusted by Holm-Bonferroni method; FDR p is the p value adjusted using False Discovery Rate; Impact is the pathway impact value calculated from pathway topology analysis.