TABLE 1.

Detection of C. jejuni and C. coli in environmental waters by PCR ELISA and selective culture on CCDA after 48 h of enrichment culture

Sample no.	Sample type	Culture result	Phenotypic identification	PCR ELISA assay resulta with:		Heat-stable serotype	TVCCb (CFU/ml)
				CC2	CJ2
1	Pond water	−		−	−
2	Pond water	+	C. coli	+	+	NTc	>1 × 107
3	Pond water	+	C. coli	+	+	NT	>1 × 107
4	Pond water	−		−	−
5	Canal water	+	C. jejuni	+	+	57	2.0 × 105
6	Pond water	+	C. coli and C. lari	+	+	NT	>1 × 105
7	Pond water	+	C. jejuni	+	+	38, 45	>1 × 107
8	Pond water	+	C. jejuni	+	+	6, 7	>1 × 107
9	Pond water	−		−	−
10	Canal water	+	C. jejuni	−	−	57	<1 × 103
11	Pond water	+	C. jejuni	−	−	NT	<1 × 103
12	Pond water	+	C. coli	+	+	NT	1.7 × 106
13	Pond water	+	C. jejuni	−	−	NT	<1 × 103
14	Pond water	+	C. coli	+	+	45	2.4 × 108
15	Brook water	−		−	−
16	Brook water	−		−	−
17	Brook water	−		−	−
18	Pond water	+	C. coli	+	−	39	5.0 × 107
19	Pond water	+	C. coli	+	−	49	180
20	Brook water	−		−	−
21	Pond water	+	C. coli	+	+	NT	3.2 × 108
22	Pond water	+	C. coli	+	+	9	7.2 × 107
23	Pond water	−		−	−
24	Pond water	+	C. coli	+	−	NT	<1 × 103
25	Canal water	−		−	−
26	Pond water	−		−	−
27	Canal water	+	C. coli	+	−	44, 48	2.0 × 107
28	Pond water	+	C. coli	+	−	NT	4.0 × 107
29	Lake water	−		−	−
30	Lake water	−		+	+
31	Lake water	−		−	−
32	Pond water	+	C. coli	+	+	20	1.8 × 108
33	Pond water	+	C. jejuni	+	+	NT	3.1 × 108
34	Pond water	+	C. jejuni	+	+	1, 17	5.0 × 107
35	Pond water	+	C. coli	+	+	3	300
36	Brook water	−		−	−
37	Canal water	+	C. coli	+	+	38, 39	1.0 × 105
38	Pond water	+	C. jejuni	+	−	NT	9.8 × 106
39	Canal water	+	C. coli	+	−	44	5.0 × 106
40	Pond water	+	C. jejuni	+	+	NT	2.6 × 108
41	Brook water	+	C. jejuni	+	+	40	1.9 × 106
42	Pond water	+	C. coli	+	−	39	2.2 × 107
43	Pond water	+	C. coli	+	−	50, 49	100
44	Brook water	−		−	−
45	Pond water	+	C. coli	+	+	NT	2.3 × 108
46	Brook water	−		−	−
47	Brook water	−		−	−
48	Canal water	+	C. coli	+	−	NT	4.0 × 105
49	Pond water	+	C. coli	+	−	50, 49	20
50	Pond water	+	C. coli	+	−	NT	3.1 × 105
51	Pond water	−		−	−
52	Pond water	+	C. coli	+	+	NT	7.0 × 107
53	Pond water	+	C. coli	+	+	NT	4.2 × 102
54	Pond water	+	C. coli	+	+	NT	1.0 × 108
55	Canal water	−		−	−
56	Brook water	+	C. coli	−	−	2	9.4 × 104
57	Pond water	+	C. coli	+	+	45	2.3 × 108
58	Lake water	+	C. coli	+	+	NT	1.2 × 108
59	Lake water	+	C. jejuni	−	−	NT	580
60	Lake water	+	C. coli	+	+	60	1.2 × 108
61	Seawater	+	C. jejuni	−	+	NT	NDd
62	Seawater	−		−	−
63	Seawater	−		+	−
64	Seawater	−		+	−
65	Seawater	−		−	−
66	Dock water	−		−	−
67	Dock water	−		+	−
68	Dock water	+	C. coli	+	+	NT	ND
69	Dock water	−		+	−

^aAt 48 h, samples were tested by PCR ELISA with CC2 and CJ2 probes. Positive (+) and negative (−) results were as indicated.

^bTVCC after 48 h of enrichment culture.

^cNT, not typeable.

^dND, not determined. No colonies were recovered on the TVCC plates.