TABLE 2.
E. coli cell type | Mean fraction retained ± SDa at:
|
||||
---|---|---|---|---|---|
0.0044 cm s−1 at:
|
0.044 cm s−1 at:
|
||||
I = 0.2 M | I = 0.02 M | I = 0.2 M | I = 0.02 M | ||
Wild-type | 0.47 ± 0.09b | 0.43 ± 0.18 | 0.70 ± 0.08b | 0.82 ± 0.09 | |
Paralyzed | 0.75 ± 0.02b | 0.78 ± 0.1∗ | 0.45 ± 0.07b∗ | 0.74 ± 0.08c | |
Smooth swimming | 0.21 ± 0.04∗ | 0.14 ± 0.007 | 0.15 ± 0.04∗ | -∗ | |
Tumbly | 0.62 ± 0.04c | -∗ | 0.65 ± 0.12c | -∗ |
-, No attached cells were observed on the glass surface during the experiment (∼1.5 h), and therefore a fraction retained could not be determined. ∗, Statistically different than the wild type (for the same conditions). I, ionic strength.
Data for wild-type and paralyzed E. coli strains in 0.2 M buffer are from McClaine and Ford (26).
We report a fraction retained, but note that the number of cells that attached during experiments ranged between 8 and 15 cells per field (and thus the number of detached cells was small). In typical experiments, the number of cells per field is between 60 and 80.