TABLE 1.
PCR amplification primers used to characterize the 13C- and 12C-DNA fractions
| Amplified gene(s) | Primers | Product (bp) | Library constructeda | Reference |
|---|---|---|---|---|
| 16S rRNA of Bacteria | Eubac27F | 1,450 | 13C-DNA (100) | 13 |
| 1492R | 12C-DNA (50) | |||
| 16S rRNA of Archaea | Arch21F | 1,450 | NAb | 13 |
| 1492R | ||||
| 18S rRNA of Eucarya | EukF | 1,500 | NA | 13 |
| EukR | ||||
| pmoA and amoA (putative active site subunit of pMMO and AMO) | A189 | 525 | 13C-DNA (50) | 23 |
| A682 | 12C-DNA (50) | |||
| pmoA (pMMO active site subunit) | A189 | 500 | 13C-DNA (50) | 11 |
| mb661 | ||||
| mxaF (MDH active site subunit) | mxa f1003 | 550 | 13C-DNA (50) | 30 |
| mxa r1561 | ||||
| mmoX (sMMO active site subunit) | mmoX F356 | 350 | 13C-DNA (10) | 16 |
| mmoX R898 |
a
DNA template used for PCR amplification and number of clones in the library (in parentheses).
b
NA, not applicable.