TABLE 1.

Strains of Xanthomonas spp. used to evaluate three set of primers, based on plasmid (2/3), virulence (J-pth), and ribosomal (J-Rx) sequences, for PCR identification

Taxon	Strain(s)	Origin	CBC type	PCR result
				2/3 primersa	J-pth primersb	J-Rx primersc
X. axonopodis pv. citri	12971	Argentina	A	+e	+	+
	12758, 12789	Bolivia	A	+	+	+
	12834, 12917	Brazil	A	+	+	+
	JH410-5, JH35, JH81.2, JJ36.2	People's Republic of China	A	+	+	+
	LW	Florida	Aw	−	+	+
	MA, MI	Florida	A	+	+	+
	CFBP1209	Hong Kong	A	+	+	+
	CFBP2851, CFBP2852	India	A	+	+	+
	X169	India	A*	+	+	+
	X406	Iran	A*	+	+	+
	CFBP2854, JJ201.2, X62, X63	Japan	A	+	+	+
	JJ37.2, JK146.4	Malaysia	A	+	+	+
	JJ9.3, JJ9.8, JK176-1	Mauritius Island	A	+	+	+
	X205	Oman	A*	+	+	+
	X100	Pakistan	A	+	+	+
	08946	Paraguay	A	+	+	+
	JJ223.2, JK148.4	Philippines	A	+	+	+
	JA151.1, B24, C31, C40	Reunion Island	A	+	+	+
	JJ10.1, JJ10.3, JJ10.6	Rodrigues Island	A	+	+	+
	X271, X292, X322, X289	Saudi Arabia	A*	+	+	+
	CFPB2548, CFBP2549, JJ53-3	Taiwan	A	+	+	+
	JK144.6, JK20.3	Thailand	A	+	+	+
	12970	Uruguay	A	+	+	+
	JN551.2	Vietnam	A	+	+	+
X. axonopodis pv. aurantifolii	X148, X69	Argentina	B	−	+	−
	X64	Argentina	B	−	−	−
	X80, X84	Uruguay	B	−	+	−
	X2905, X2906, X341, X70	Brazil	C	−	+	−
X. axonopodis pv. citrumelo	F1	Florida	NAd	−	−	−
X. axonopodis pv. dieffenbachiae	X462, X175a, X175b, X376, X696	Florida	NA	−	−	−

^aThe 2/3 primers were designed to amplify inside the X. axonopodis pv. citri plasmid (8).

^bPrimers for the pth gene (primers J-pth1 and J-pth2).

^cPrimers for rDNA (primers J-RXg and J-RXc2).

^dNA, not applicable.

^e+, fragment of expected size amplified; −, fragment of the expected size not amplified.