Figure 2.

CleanBar barcode detection results. (A) CleanBar screen output. Example of a sequence (number 46) showing exact alignment of each of the four barcode groups (D, C, B, A) in both direct (dir) and reverse complement (rc) orientations. The output displays the barcode position, barcode exact alignment, and its label. (B) Linkers length. Number of linkers with lengths equal to 12 or shorter or longer than 12 in relation to the barcode end position. Nearly all barcodes have a length of 12. Barcodes detected further from the beginning of the sequence do not have longer linkers between them. (C) Total read counts per barcode. Number of reads with a given barcode detected at a given position of the plate.