Table 2.

Metabolism of ADQ by individual human CYPs.^a

	pmol metabolite/mg microsomal protein/min
	NADQ	AADQ
HepG2	0.74 ± 0.05	0.32 ± 0.04
CYP vector	1.91 ± 0.01	0.24 ± 0.08
CYP1A1	58.9 ± 1.90*	54.9 ± 2.10*
CYP1A2	11.2 ± 0.42*	0.54 ± 0.01
CYP1B1	15.4 ± 0.32*	13.0 ± 0.71*
CYP2A6	3.66 ± 0.28*	0.21 ± 0.02
CYP2A7	0.26 ± 0.08	0.19 ± 0.01
CYP2A13	3.73 ± 0.05*	0.17 ± 0.01
CYP2B6	2.71 ± 0.19*	0.23 ± 0.04
CYP2C8	106 ± 6.90*	0.27 ± 0.02
CYP2C9	0.68 ± 0.04	0.25 ± 0.03
CYP2C18	2.33 ± 0.13*	0.24 ± 0.02
CYP2C19	11.9 ± 0.32*	0.17 ± 0.01
CYP2D6	7.76 ± 0.37*	0.39 ± 0.04*
CYP2E1	3.47 ± 0.11*	0.19 ± 0.02
CYP3A4	29.6 ± 1.90*	1.05 ± 0.02*
CYP3A5	14.5 ± 0.75*	0.79 ± 0.07*
CYP3A7	2.96 ± 0.12*	0.20 ± 0.03
CYP4A11	0.57 ± 0.13	0.16 ± 0.05
CYP4B1	0.98 ± 0.13	0.15 ± 0.02

^aMetabolism of ADQ by microsomes isolated from HepG2 cells overexpressing human CYPs was assayed in a 125 μl final reaction volume by incubating microsomes (1.0 mg of microsomal protein) with 0.8 mM ADQ, 4 mM MgCl₂, 2 mM NADPH, and 100 mM potassium phosphate buffer (pH 7.2) at 37 °C for 30 min with gentle shaking. The metabolites were analyzed using HPLC-MS/MS as described in Materials and Methods. Data are presented as the mean ± standard deviation.

^*Significantly (p < .05) different from the CYP vector cell line.