Table 4.
IC50 values of ADQ in HepG2 cell lines overexpressing CYP2C8 and 3A4.a
| IC50 (μM) | 95% confidence intervals (μM) | |
|---|---|---|
| CYP Vector | 18.9 | 16.3–22.0 |
| CYP2C8 | 11.6 | 9.8–13.7 |
| CYP2C8 + Rifampicin | 17.9* | 16.2–19.7 |
| CYP3A4 | 14.8 | 13.0–16.9 |
| CYP3A4 + Ketoconazole | 19.6* | 17.2–22.4 |
HepG2 cell lines overexpressing CYP2C8 and 3A4 were incubated with various concentrations of ADQ (3.13–50 μM) in the presence or the absence of the CYP inhibitor (30 μM rifampicin or ketoconazole) for 48 h. The IC50 values were obtained from the cell growth curves, using GraphPad Prism 6.0. Data are presented as the mean IC50 of three independent experiments and 95% confidence intervals of the IC50.
Significantly (p < .05) different from cells treated without the CYP inhibitor.