Table 2.

Examples of DMSO-free cryopreservation of NK and T cells.

Cell type	CPA	Post-thaw function
NK-92	Trehalose encapsulated in chitosan-TPP nanoparticles	Viability comparable to DMSO CPA; higher cytotoxicity compared to DMSO CPA[141]
NK-92	7.5% COOH-PLL + 5% dextran-40 + ectoine	Viability comparable to DMSO CPA[169]
NK-92	5% Dextran-40 + 7.5% COOH-PLL	Viability and cytotoxicity comparable to DMSO-based CPA[205]
Jurkat T cells	300 mM trehalose, 10% glycerol and 0.01% ectoine	Higher viability compared to DMSO CPA[202]
Jurkat T cells	Sucrose + glycerol + isoleucine	CPA optimization study; no direct comparison to DMSO CPA[203]
Jurkat T cells	Sucrose + glycerol; Trehalose + glycerol	Improved post-thaw function compared to DMSO[204]
PBMC-derived helper T cells; cytotoxic T cells; NKT cells	Sucrose + glycerol + isoleucine (SGI); Trehalose + glycerol + isoleucine (TGI); Maltose + glycerol + isoleucine (MGI)	Comparable recovery for helper and cytotoxic T cells to DMSO CPA; highest recovery with helper T cells in TGI; poor recovery for NKT cells[111]

COOH: carboxylated poly-L-lysine; CPA: cryoprotective agent; DMSO: dimethylsulfoxide; NK: natural killer