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. Author manuscript; available in PMC: 2025 Aug 25.
Published in final edited form as: Toxicology. 2025 Jan 31;512:154068. doi: 10.1016/j.tox.2025.154068

Fig. 1.

Fig. 1.

Apoptosis is observed in mouse Sertoli TM4 cells after CBD exposure for 48 h. Mouse Sertoli TM4 cells were treated with DMSO or the indicated CBD concentrations for 24 h or 48 h. (A) Representative scatter plots show the flow cytometric results for Annexin V/PI staining after CBD treatment for 24 h and 48 h. (B & C) Quantification of Annexin V/PI staining results. Bar graphs show the mean percentage of viable, early apoptotic, or late apoptotic cells ± SD (n = 3). *, p < 0.05, significantly different from the DMSO control.