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. Author manuscript; available in PMC: 2025 Aug 25.
Published in final edited form as: Toxicology. 2025 Jan 31;512:154068. doi: 10.1016/j.tox.2025.154068

Fig. 2.

Fig. 2.

CBD inhibits cellular growth and induces apoptosis after longer-term incubation in mouse Sertoli TM4 cells. Mouse Sertoli TM4 cells were treated with DMSO, 15 μM CBD, or 20 μM CBD for 6 days. (A) The line graph shows the cumulative population doubling levels (n = 3). (B) The bar graph shows the relative fluorescence intensity of SA-β-gal in mouse Sertoli TM4 cells treated with DMSO or 20 μM CBD. The bar graph represents means ± SD (n = 3). (C) Representative scatter plots show the flow cytometric results for Annexin V/PI staining after DMSO, 15 μM CBD, or 20 μM CBD treatment for 3 or 6 days. (D) Quantification of Annexin V/PI staining results. Bar graphs show the mean percentage of viable, early apoptotic, or late apoptotic cells ± SD (n = 3). *, p < 0.05, significantly different from the DMSO control.