Table 1.
Relative cellular growth level measured using Cell Titer-Blue assay.a.
| CBD (μM) | Cellular growth in mouse Sertoli TM4 cells | CBD (μM) | Cellular growth in primary human Sertoli cells |
|---|---|---|---|
| 10 | 92.3 ± 8.2 %# | 7 | 101.5 ± 3.9 %# |
| 12.5 | 91.1 ± 7.6 % | 8 | 91.7 ± 3.9 %* |
| 15 | 91.6 ± 9.9 % | 9 | 76.2 % ± 3.3 %* |
| 17.5 | 84.3 ± 11.1 % | 10 | 65.2 ± 2.1 %* |
| 20 | 73.2 % ± 6.3 %* |
a
These results were obtained from our previous study (Li et al. 2022). Mouse Sertoli TM4 and primary human Sertoli cells were incubated with various concentrations of CBD for 24 h. Cytotoxicity was determined using CellTiter-Blue assy. The percentage of cellular growth was calculated from normalization to the control group.
#
significant concentration-related linear trend.
*
significantly different from the DMSO control.