FIG. 2.

(A) RFLP analysis of HaeIII-restricted nifH amplicons from DNA extracted from composite bulk and rhizosphere soil obtained at the S. patens and P. australis sites at a depth of 2.5 cm during major plant phenological stages, including dormancy (lanes D), vegetative growth (lanes V), reproduction (lanes R), and senescence (lanes S). The size marker (lanes M) was pUC19 cleaved with Sau3A. (B) Corresponding UPGMA dendrogram based on analysis of fragments that were between 105 and 258 bp long. If the level of similarity between patterns was greater than 0.7, the patterns were not considered different. Sp, S. patens; Pa, P. australis; D, dormancy; V, vegetative growth; R, reproduction; S, senescence.