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. 2002 Mar;68(3):1228–1231. doi: 10.1128/AEM.68.3.1228-1231.2002

FIG. 2.

FIG. 2.

Protein analysis of Cyt2Bc-containing inclusions. (A) Purified inclusions corresponding to 10 μg of protein were subjected to electrophoresis in 15% polyacrylamide gels containing SDS and stained with Coomassie brilliant blue. Lane 1, Cyt2Bc; lane 2, Cyt2Ba; lane 3, Cyt1Aa. (B) Purified inclusions were subjected to electrophoresis (as described above) and then transferred onto a nitrocellulose filter. Incubation with antiserum raised against either Cyt2Ba (a) or Cyt1Aa (b) and detection of immunoreactive polypeptides were performed as described in Materials and Methods. Lane 1, Cyt2Bc (1 μg); lane 2, Cyt2Ba (0.1 μg); lane 3, Cyt1Aa (5 μg); lane 4, Cyt2Bc (5 μg); lane 5, Cyt2Ba (5 μg); lane 6, Cyt1Aa (0.05 μg). Molecular mass is indicated in kilodaltons at the left of panel A and in the center of panel B.