TABLE 1.
Hemolytic activities of solubilized and activated Cyt2Bc toxina
| Treatmentb | Activity of toxin:
|
||
|---|---|---|---|
| Cyt2Bc | Cyt2Ba | Cyt1Aa | |
| Solubilization | >390c | 31.2 (25.9-37.5) | 0.147 (0.138-0.157) |
| Proteinase K | |||
| 1 h | >200c | >250c | 0.058 (0.053-0.063) |
| 2 h | >200c | >250c | 0.064 (0.059-0.069) |
| 14 h | >200c | >250c | 0.054 (0.051-0.058) |
| Trypsin | |||
| 1 h | 9.7 (8.5-11.5) | 3.0 (2.7-3.3) | 0.023 (0.021-0.024) |
| 2 h | 8.5 (7.6-9.6) | 1.4 (1.3-1.6) | 0.034 (0.032-0.036) |
| 14 h | 4.5 (4.2-4.9) | 0.9 (0.8-0.9) | 0.026 (0.025-0.027) |
a
Doses giving 50% hemolysis are expressed in micrograms of protein. Values are the means of at least three experiments. Numbers in parentheses are 95% confidence limits, as determined by Probit analysis.
b
Proteins were solubilized and activated as described in Materials and Methods.
c
At this concentration, no hemolytic activity was detected.