Table 1.

BBD for optimization of the immobilization process

Run	Beads conc. (rise husk concentration, X1)	Enzyme (U/g, X2)	Time (h, X3)	Immobilized yield (%)		Residual
				Actual value	Predicted value
1	1(0)	2.25(0)	5(0)	62.37 ± 1.14	58.15	4.22
2	1(0)	2.25(0)	5(0)	62.01 ± 1.60	58.15	3.86
3	0.5(-)	1.75(-)	5(0)	96.45 ± 0.39	96.23	0.2175
4	1.5(+)	2.25(0)	6(+)	56.89 ± 0.14	57.41	-0.5150
5	1(0)	2.25(0)	5(0)	43.48 ± 1.32	58.15	-14.67
6	1.5(+)	1.75(-)	5(0)	69.38 ± 2.26	69.92	-0.5425
7	0.5(-)	2.25(0)	4(-)	93.44 ± 2.02	92.92	0.5150
8	1(0)	2.75(+)	4(-)	71.45 ± 2.82	72.51	-1.06
9	1(0)	2.25(0)	5(0)	61.34 ± 1.25	58.15	3.19
10	1(0)	2.25(0)	5(0)	61.55 ± 0.72	58.15	3.40
11	0.5(-)	2.25(0)	6(+)	76.85 ± 1.51	78.13	-1.28
12	1(0)	2.75(+)	6(+)	78.58 ± 1.41	77.85	0.7325
13	1.5(+)	2.75(+)	5(0)	95.62 ± 0.26	95.84	-0.2175
14	1.5(+)	2.25(0)	4(-)	95.33 ± 0.21	94.06	1.27
15	0.5(-)	2.75(+)	5(0)	89.66 ± 2.31	89.12	0.5425
16	1(0)	1.75(-)	4(-)	93.44 ± 0.41	94.17	-0.7325
17	1(0)	1.75(-)	6(+)	38.44 ± 1.97	37.38	1.06