Skip to main content
. 2002 Mar;68(3):1290–1296. doi: 10.1128/AEM.68.3.1290-1296.2002

FIG. 2.

FIG. 2.

Negative images of gel electrophoresis of PCR fragments amplified in second-stage PCR. All lane numbers correspond to those listed in Table 1. Lane M was loaded with the products of the modified reaction (SIB99 M) using only the upstream primer AVS1. (A) PCR products were electrophoresed in 1.5% LE agarose in 1× TAE buffer. Numbers to the left of the gel correspond to the size of standardsloaded in lanes L, and lane N was loaded with the negative control. (B) The lines specify the position of all bands excised from the gel. (C) Lines indicate the 14 bands, SO98-1, SO98-2, SO98-3, SO98-5, BSA99-1, BSA99-2, BSA99-5, BSA99-9, BSB99-2, SIA99-1, MIB99-2, PSB99-1, PSC99-1, and PSC99-2, that produced unique pol-like sequences (solid lines) and a band, BSA99-9, that did not produce a pol-like sequence (broken line).