Table 1.
Primers used
| primer | sequence (5’−3’) | position |
|---|---|---|
| hitA_F | TTGAGGCACAGCAATAGC | 439 269–439 2861 |
| hitA_R | AAGGCTTAGGTAAGGAATGATTAG | 439 204–439 2271 |
| gap_F | GCAGGCTCAATATTTGACTCACT | 660 140–660 1621 |
| gap_R | GATGATTCATTGTCGTATCATGC | 660 212–660 2341 |
| lgt_F | TGAAATTGATTACGTCCAGGAA | 420 553 − 420 5741 |
| lgt_R | CCGAAACGAATTATTCCATAATAAAC | 420 507 − 420 5321 |
| upXRE_F1 | CGACATATTTTATTAAAGACTTAAAACTTATT | 497–5282 |
| upXRE_F2 | GATATGCCATGGTATTAATCAGTTAT | 571–5962 |
| XRE_F | GAGAATTGACCGGATTAGGAACR | 649–6712 |
| XRE_R | CTCCACCACATCTTCTATTTTACAATTT | 743–7702 |
| M1_R1 | CTATTAAAATATAATTAAAATCATTATCTTCA | 1224–12512 |
| M1_F | GAGTTCACACTGCACAACATATCTTAGTT | 1451–14792 |
| M1_R2 | TTTTTGGATAGAAGACAGGATTTTC | 1513–15372 |
| M1_F2 | ATGAAATTACTGGTGAAAGACTAAAAG | 1538–15642 |
| M2_R1 | TCTTCAATAATATCCATTACATAAAAAAC | 2156–21842 |
| M2_F | GGATGGATCTCCAATTAAAGGTTTT | 2596–26202 |
| M2_R2 | CCACCCATATTGCCATTATTCA | 2673–26942 |
| R_F | TTCGCTTTAGTCAAATGTGCTTTT | 3113–31362 |
| R_R | AGGATGTGGATATTGCAAAGCAA | 3245–32672 |
| downR_F | GTATAGTTGTTGCTCCACGTTTCTCAC | 4172–41982 |
| downR_R1 | GCTGTATTGATTATTGTTTTTTTCAT | 4448–44732 |
| downR_R2 | ACGACCTAACTCATAATTGAGCCAAG | 4483–45082 |
1Position in M. hominis PX1114 (acc.-no. CP032849.1)
2Position relative to the mhoVI-gene cassette in PX1114 (pos. 391 805–386 982; see Fig. 1)