TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| HB101 | supE44 hsdS20 (rB− mB−) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 | Takara, Osaka, Japan |
| JM109 | recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB) | |
| F′ [traD36 proAB+lacIqlacZΔM15] | Takara, Osaka, Japan | |
| Rhizobium sp. | ||
| AC100 | Wild type | This study |
| AC101 | Car− | This study |
| AC1012 | Car− Strr Rifr | This study |
| Plasmids | ||
| pRK2013 | Kmrtra+; triparental mating helper, ColE1 replication | 13 |
| pBI101 | Kmr; broad-host-range cloning vector | Clontech |
| pBluescript II SK(+) | Ampr; sequencing vector | Stratagene |
| pCEH-B | 23-kb BamHI fragment from pAC200 cloned into pBI101 | This study |
| pCEH-Sa | 6.2-kb SalI fragment from pAC200 cloned into pBI101 | This study |
| pCEH-Sm | 7.2-kb SmaI fragment from pAC200 cloned into pBI101 | This study |
| pCEH-EVB | 3.2-kb EcoRV-BamHI fragment from pCEH-B subcloned into pBI101 | This study |
| pCEH-EVSc | 2.7-kb EcoRV-SacII fragment from pCEH-B subcloned into pBI101 | This study |