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Purification of the carbaryl hydrolase
| Purification step | Total activity (U) | Total protein (mg) | Sp act (U/mg) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
| Crude extract | 69.3 | 1,520 | 0.05 | 1.0 | 100 |
| Protamine treatment | 69.4 | 953 | 0.07 | 1.4 | 100 |
| Ammonium sulfate (25-50%) | 72.1 | 262 | 0.27 | 5.6 | 104 |
| Q Sepharose | 114 | 12.3 | 9.30 | 185 | 165 |
| SP Sepharose | 78.8 | 3.9 | 20.2 | 404 | 114 |
| Gel filtration | 18.4 | 0.6 | 30.7 | 614 | 27 |
