Figure 2.

Identification of thyrocyte heterogeneity between paracancerous tissues and tumors at varying progression levels. A) UMAP plot depicting distinct clusters based on gene expression differences for 14965 thyrocytes that passed quality control. B) Feature plots displaying the normalized expression of highly expressed genes in each thyrocyte subcluster. C) UMAP projection visualizing the distribution of thyrocytes across tissues, color‐coded by tissue type. D) Violin plots showing the TDS score distribution for each thyrocyte cluster. E) Bar plots indicating the proportion of eight thyrocyte subclusters across each stage. F) Mapping of the three thyrocyte clusters identified through single‐cell sequencing onto tissue slices. G) Monocle pseudotime trajectory analysis of thyrocytes using highly variable genes, with each dot representing a single cell, colored according to its cluster label. H) Heatmap displaying the top 10 differentially expressed genes across the eight thyrocyte clusters. I) Heatmap showing the 50 hallmark pathways (rows) significantly enriched in thyrocytes for each stage (columns).