FIG. 5.

SDS-PAGE of proteins of S. meliloti strain 1080. Proteins were isolated from cells extracted from peat cultures 2 months (lanes 1 and 2) and 14 days (lanes 3 and 4) after inoculation and cells from stationary-phase broth cultures (lanes 5 and 6). Electrophoresis was performed in 12% (wt/vol) polyacrylamide gels, and proteins were stained with Coomassie brilliant blue R250. The amounts of protein loaded onto the lanes were 100 (lanes 1, 3, and 5) and 50 (lanes 2, 4, and 6) μg. The arrow indicates the polypeptide band that was excised for N-terminal sequencing.