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. Author manuscript; available in PMC: 2025 Aug 25.
Published in final edited form as: Nat Methods. 2024 Nov 8;22(1):24–40. doi: 10.1038/s41592-024-02480-7

Figure 1.

Figure 1.

hiPSC culture and differentiation. a. Histogram of the number of control hiPSC lines publications reported using for hiPSC-CM differentiation. b. Top 15 reported hiPSC lines in the analyzed publications. c. Histogram of the reported purity of cardiomyocytes after hiPSC-CM differentiation. Inset: The reported methods of assessing the purity of cardiomyocytes after hiPSC-CM differentiation. d. Schematic of the variability in hiPSC-CM differentiation protocols. Confluency of hiPSCs is typically at ~80–90% at the start of differentiation (Day 0) (n = 108). There is variability in the time course of Wnt pathway activation (green) and inhibition (blue) in hiPSC-CM differentiation, along with when insulin is added to the media (pink) and the hiPSC-CM purification method (red). Pie charts represent the top reported Wnt pathway activators (n = 229), Wnt pathway inhibitors (n = 219), and purification methods (n = 300). n values represent the number of publications that utilized and reported on each differentiation component.