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Validation of quantitative mass spectrometry analysis. The western blotting analysis demonstrates the brain expression level of protein candidates, 14–3-3 (pan) and 14–3-3 gamma isoform, in aged male subjects (A) and aged female subjects (B) after ICH or sham. (C & D) and (E &F) illustrate the densitometry analysis of the western blotting data normalized against beta-actin from aged male and female subjects, respectively (n = 4–5/group).
