Table 2. Estimation of the binding constants (K_b) at three varied temperatures and thermodynamic parameters of ct-DNA–TAF complex.

T (K)	K b (M−1)	ΔH° (kJ mol−1)	ΔS° (J mol−1 K−1)	ΔG°a (kJ mol−1)	ΔG°b (kJ mol−1)
298	2.2 × 105	6.23	123.48	−30.57	−30.47
303	2.4 × 105			−31.17	−31.18
308	2.5 × 105			−31.80	−31.88

^aΔG° = RT ln K_b.

^bΔG° = ΔH° − TΔS°.