FIG. 2.
Genetic organization of the cloned region including the acrM gene and that of the disruptant. (A) ORFs and restriction map of the cloned BamHI-HindIII 4.0-kb cloned fragment. (B) Organization of the disruption vector, pDAR1, derived from pBM4. The hatched box represents the kanamycin resistance gene. (C) Genomic Southern analysis of DraI-digested total DNA (5.0 μg per each lane) from the wild-type strain (lane 1) and the acrM-KO strain (lane 2), using the 32P-labeled acrM gene fragment as a probe.