Table 1.
Primers and probes used for GM maize event-specific targets and the endogenous reference gene (hmg).
| Event name | Primers/probes sequence (5’-3’) | aLength (bp) | |
|---|---|---|---|
| DAS01131–3 | F- CTA AGA GCT AAG ATT GCG CGG R- TTC GGG CCT AAC TTT TGG TG p- [6-FAM]-ACA TAT TTT TTG AGG ATA ACA GCA-[NFQ-MGB] |
98 | |
| DAS40278–9 | F- CAC GAA CCA TTG AGT TAC AAT C R- TGG TTC ATT GTA TTC TGG CTT TG p- [6-FAM]- AGC TAA CCT TCA TTG TAT TCC G-[TAMRA] |
98 | |
| DAS59122–7 | F- GGG ATA AGC AAG TAA AAG CGC TC R- CCT TAA TTC TCC GCT CAT GAT CAG p- [6-FAM]- TTT AAA CTG AAG GCG GGA AAC GAC AA-[TAMRA] |
86 | |
| DP 004114–3 | F- CGT TTG TAG CAC TTG CAC GTA GT R- GGT AAC CGC TCT TCC AGT TGA A p- [6-FAM]- AAG CTT CAA CAC AGA TC-[NFQ-MGB] |
90 | |
| DP023211–2 | F- CAT TTT GGA TTG TAA TAT GTG TAC CTC A R- CTA GCT CGA CTA GTT AGT TAG ACG CG p- [6-FAM]- TTA AAT CTG ACG TGA GGC GC-[NFA-MGB] |
92 | |
| DP098140–6 | F- GTG TGT ATG TCT CTT TGC TTG GTC TT R- GAT TGT CGT TTC CCG CCT TC p- [6-FAM]- CTC TAT CGA TCC CCC TCT TTG ATA GTT TAA ACT-[TAMRA] |
80 | |
| DP202216–6 | F- CCA TCT GAG GTC TGC ACT CTC AC R- CTC CGC TCA TGA TCA GAT TGT C p- [6-FAM]- CAA CAC ACT CAA ACA CTG ATA G-[NFQ-MGB] |
105 | |
| MON87403–1 | F- CTT TCT TTT TCT CCA TAT TGA CCA TCA TAC R- TAC TCC GGA ATG AGT GCT CTG TAT C p- [6-FAM]- TCA TTG CGA TCC ACA TTT CCC TAC ATG G-[TAMRA] |
88 | |
| MON87411–9 | F- CTC TGT AAC AGA AAA CAC CAT CTA GAG R- ACA AAA GTG AAC TAG TTC TAG GGT AGA T p- [6-FAM]- CCG CGT TTA AAC TAT CAG TGT TTA GAG AAT-[TAMRA] |
109 | |
| MON87429–9 | F- CGA GAC AGA CTC AAT GTA TCC GAG ATA CTC R- CCA TCA TAC TCA TTG CTG ATC CAT GTA p- [6-FAM]- TCC CGG ACA TGA AAC CAA ACA AGA GTG GTC-[TAMRA] |
116 | |
| MON87460–4 | F- CAC GTT GAA GGA AAA TGG ATT G R- TCG CGA TCC TCC TCA AAG AC p- [6-FAM]- AGG GAG TAT GTA GAT AAA TTT TCA AAG CGT TAG ACG GC-[TAMRA] |
82 | |
| MON88017–3 | F- GAG CAG GAC CTG CAG AAG CT R- TCC GGA GTT GAC CAT CCA p- [6-FAM]- TCC CGC CTT CAG TTT AAA CAG AGT CGG GT-[TAMRA] |
94 | |
| hmg (endogenous reference gene) | F- TTG GAC TAG AAA TCT CGT GCT GA R- GCT ACA TAG GGA GCC TTG TCC T p- [VIC]-CAA TCC ACA CAA ACG CAC GCG TA-[NFQ-MGB] |
79 | |
aLength denotes the amplicon size in base pairs. In primer/probe sequence, F, R, and P indicate forward primer, reverse primer, and probe, respectively. TAMRA, 6-carboxytetramethylrhodamin NFQ-MGB, non fluorescent quencher-minor groove binder.