Table 1.
Strains, vectors, and primers used in this study.
| Strains | Description | Reference (s) |
|---|---|---|
| Pseudomonas syringaepv. actinidiae | ||
| G1 | Psa biovar 3 strain | [19] |
| G126 | Psa biovar 3 strain | This study |
| G282 | Psa biovar 3 strain | This study |
| G126 hrpRSC-T | Complimentary strain of G126 | This study |
| Escherichia coli | ||
| DH5α | For cloning of recombinant plasmids | |
| Plasmids | ||
| pDSK-GFPuv | Vector to construct luciferase reporter strains | [28] |
| pK18mobSacB | Vector for creating knockout strain | [29] |
| pDSK-PHrpRS:Nluc | Luciferase reporter vector of hrpRS gene promoter | This study |
| pDSK-PHrpL:Nluc | Luciferase reporter vector of hrpL gene promoter | This study |
| pDSK-HrpA:Nluc | Luciferase reporter vector of hrpA accumulation | This study |
| G1-PHrpRS:Nluc | Luciferase reporter vector of hrpRS gene promoter of G1 | [19] |
| G1-PHrpL:Nluc | Luciferase reporter vector of hrpL gene promoter of G1 | [19] |
| G1-HrpA:Nluc | Luciferase reporter vector of hrpA gene promoter of G1 | [19] |
| G126-PHrpRS:Nluc | Luciferase reporter vector of hrpRS gene promoter of G126 | This study |
| G126-PHrpL:Nluc | Luciferase reporter vector of hrpL gene promoter of G126 | This study |
| G1126-HrpA:Nluc | Luciferase reporter vector of hrpA gene promoter of G126 | This study |
| G282-PHrpRS:Nluc | Luciferase reporter vector of hrpRS gene promoter of G282 | This study |
| G282-PHrpL:Nluc | Luciferase reporter vector of hrpL gene promoter of G282 | This study |
| G282-HrpA:Nluc | Luciferase reporter vector of hrpA gene promoter of G282 | This study |
| Primers | Sequences (5”−3”) | |
| HrpR/S-C-F3 | CCGGTACCTCGGCTTGGTATGATGATATTACT | This study |
| HrpR/S-C-R2 | GCTCTAGAGGTTCCAGCGTCTTTGCA | |
| M13F | GTTTTCCCAGTCACGAC | Lab stock |
| M13R | CAGGAAACAGCTATGAC | |
| P0F | CTGCAACAGGCGACGGCGAGGC | [30] |
| P6R | CATAGGCTTCTGGTTTTCTTCCTGATCC | |
| hrpL-F | AGCCGGGTTATGTTCGC | This study |
| hrpL-R | TTGAGTCGAGGATCACAATCT | |
| hrpANLuc-F | GGTGGCTCTGGTGGCGGTGGCTCTGGTGGTATGGTCTTCACACTCGAAGATTTC | This study |
| hrpANLuc-R | TCAGAAATAATTTACGCCAGAATGCGTTCG | |