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. 2025 Aug 22;32(1):2547751. doi: 10.1080/10717544.2025.2547751

Table 1.

Summary of bioactive compounds that induce white fat browning.

Classification Browning agent Mechanism Challenges
β3-AR CL316243 (Cero et al. 2021) Activation of β3-sympathomimetic activity Poor oral bioavailability, poor gastrointestinal absorption, and first-pass hepatic destruction, side-effects (such as increased heart rate and blood pressure)
Mirabegron (Cero et al. 2021) Activation of β3-sympathomimetic activity Cardiovascular side effects
PPARγ agonist Rosiglitazone (Ohno et al. 2012) Stabilization of PRDM16 protein Off-target effects (such as body weight gain, congestive heart failure, bone loss, bone fracture)
AMPK agonist Metformin (Malin and Kashyap 2014) Activation of AMPK and FGF21 Low bioavailability, low targeting specificity, gastrointestinal side effects
Notch signaling
Inhibitor
DBZ (Bi et al. 2014) Inhibition of Notch signaling Off-target effects, large systemic dose requirement, undesired drug accumulation in the liver
TH T3 (López et al. 2010) Decreased activity of hypothalamic AMPK, increased SNS activity Multiple deleterious effects (Cardiotoxicity, bone loss, muscle wasting, perturbation of the neuroendocrine circuit), impair thermogenesis in BAT
BMPs BMP7 (Boon et al. 2013) Sympathetic activation No targeting specificity, systemic side effects
o-3 unsaturated fatty acid DHA (Zhuang et al. 2019) Increased mitochondria-mediated β-oxidation Low enrichment efficiency for adipose tissue, suboptimal bioavailability. easily destroyed by oxidation, low efficacy, unexpected side effects
Nitrogen oxides NO (Dai et al. 2013) cGMP-dependent pathways and cross-talk with other molecules such as AMPK, PPARγ, PGC-1α Low bioavailability
Natural products Cap (Baskaran et al. 2016), Res (Qiang et al. 2012), et al. Activation of TRPV1 channels, Sirt1-dependent deacetylation of PPARγ Considerable hydrophobicity, low bioavailability, obvious irritation of the mouth and gastrointestinal tract

Note: PPAR γ: peroxisome proliferator-activated receptor γ; AMPK: Adenosine 5’-monophosphate-activated protein kinase; T3: triiodothyronine; TH: Thyroid hormone; BMPs: Bone morphogenetic proteins; Bmp7: Bone morphogenetic protein 7; β3-AR: β3-adrenergic receptor; TRPV1: transient receptor potential cation channel subfamily V member 1; SIRT1: NAD-dependent deacetylase sirtuin 1; PGC1-α: peroxisome proliferator-activated receptor-gamma coactivator-1α; BAT: brown adipose tissue; PRDM16: PR domain-containing 16; FGF21: Fibroblast growth factor 21; DBZ: Dibenzazepine; DHA: Docosahexaenoic acid; SNS: Sympathetic nervous system; cGMP: Cyclic guanosine monophosphate; TRPV1: transient receptor potential cation channel subfamily V member 1; Cap, Capsaicin; Res, Resveratrol.