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A composite DIC image of an entire biofilm vertical section and CSLM projection images of the microscopic fields enclosed by boxes after in situ hybridization with TRITC-labeled SRB 385 probe and fluorescein isothiocyanate-labeled EUB338 and staining with DAPI displaying the vertical distributions of total cells, and general bacteria, and SRB385-hybridized cells in the biofilm. The biofilm sample was taken in the 8th week. The biofilm surface is at the top of each panel.
