FIG. 6.

(A) A DGGE profile of PCR-amplified 16S rRNA gene fragments obtained with a primer set of SRB385f-GC and 907R and bacterial genomic DNA extracted from a wastewater biofilm at different developmental stages. Lanes: (1) Inf, influent wastewater; (2) 1-week-, (3) 3-week-, (4) 5-week-, (5) 5.5-week-, (6) 6 week-old biofilm; (7) Dbl: Desulfobacter latus; (8) Dvs: Desulfovibrio salexigens, and (9) Dbp: Desulfobulbus propionicus. The numbers next to the arrows refer to the numbers of excised and successfully sequenced bands. (B) Phylogenetic analysis of the partial 16S rDNA sequences (461 bp) derived from the excised DGGE bands depicted in Fig. 6A. The trees were calculated by neighbor-joining algorithm and a 50% conservation filter. The scale bar denotes 5% sequence divergence, and the values at the nodes represent bootstrap values (resampling analysis performed 100 times).