FIG. 1.

Identities and phenotypes of the S. oneidensis mutants. (A) The biofilm mutant and the iron reduction mutant were generated by transposon insertion (indicated by triangles) into the fliI gene and the menC gene, respectively. These genes lie on different parts of the chromosome, and the flanking regions on either side of the disrupted genes are shown. The arrow indicates the direction of transcription for all open reading frames. The open reading frames are labeled on the basis of homology to known E. coli genes. hypoth, hypothetical gene. (B) Attachment of the fliI and menC mutants and the wild type (WT) to 1018 steel after 24 h. Light spots are DAPI-stained cells. Bar = 10 μm. (C) Reduction of goethite to Fe(II) over time for representative cultures. Symbols: □, wild type; ◊, fliI mutant; ○, menC mutant.