Skip to main content
. 2025 Jun 25;8:0860. doi: 10.34133/research.0860

Fig. 2.

Fig. 2.

GLA exposure induces ferroptosis in mouse testes. The Fe2+ content of testes tissues (A). The protein expression levels of GPX4, SLC7All, and ACSL4 in testes tissues (B). The Fe2+ content of Sertoli cells and Leydig cells (C). Representative fluorescence images of lipid peroxidation assay in Sertoli cells and Leydig cells following GLA treatment for 24 h combined with Fer-1 (200 nM) or DFP (6 or 4 μM) (D). The protein expression levels of GPX4, SLC7All, and ACSL4 of Sertoli cells and Leydig cells (E). MDA content (F). Representative fluorescence confocal images of mROS with MitoSox dye in Sertoli cells and Leydig cells following GLA treatment for 24 h combined with Fer-1 or DFP (G). The protein expression levels of GPX4, SLC7All, and ACSL4 in Sertoli cells and Leydig cells following GLA treatment for 24 h combined with Fer-1 or DFP (H). Data are presented as mean ± SD of at least 3 independent experiments. “*” indicates significant difference compared with the control group (*P < 0.05, **P < 0.01, and ***P < 0.001). “#” indicates significant difference between the GLA treatment group and combined treatment group (#P < 0.05 and ###P < 0.001).