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. 2025 Jun 25;8:0860. doi: 10.34133/research.0860

Fig. 3.

Fig. 3.

GLA exposure triggers mitophagy in mouse testes. The protein expression levels of ATG5, LC3B, and SQSTM1 in testes tissues (A) and Sertoli cells and Leydig cells (B). Representative fluorescence confocal images of endogenous LC3B in Sertoli cells and Leydig cells following GLA treatment for 24 h combined with 3-MA (60 or 90 μM), CQ (0.5 mΜ or 4 μM), or Baf-A1 (12 or 4 nM) and statistical analysis (C). The protein expression levels of ATG5, LC3B, and SQSTM1 in Sertoli cells and Leydig cells following GLA treatment for 24 h combined with 3-MA, CQ, or Baf-A1 (D). The protein expression levels of ATG5, LC3B, and SQSTM1 in Sertoli cells and Leydig cells following GLA treatment for 24 h combined with Fer-1or DFP (E). “*” indicates significant difference compared with the control group (*P < 0.05, **P < 0.01, and ***P < 0.001). “#” indicates significant difference between the GLA treatment group and combined treatment group (#P < 0.05, ##P < 0.01, and ###P < 0.001).