| α-MEM medium |
gibco/Thermo Fisher Scientific |
12571-063 |
| 10% FBS |
Merck |
F4135 |
| 3-Isobutyl-1-methylxanthine (IBMX) |
Sigma Aldrich/Biogems |
I7018/288542-100MG |
| commercial adipocyte differentiation media |
iXCells |
MD-0005 |
| LipidSpot |
Fisher Scientific |
NC1669425 |
| Hoescht 33342, trihydrochloride, trihydrate |
invitrogen/ThermoFisher Scientific |
3570 |
| UCP1 |
Abcam |
AB10983 |
| Cultrex® Mouse Laminin I |
R&D Systems/biotechne |
3400-010-02 |
| Fibronectin Human, Natural |
Sigma-Aldrich |
F4759-5MG |
| poly-L-ornithine |
Sigma-Aldrich |
P2533 |
| Ciliary Neurotrophic Factor |
R&D Systems |
257-NT-050-CF |
| Neuregulin |
R&D Systems |
396-HB-050/CF |
| S100β |
Abcam |
AB52642 |
| SOX10 |
Gift of Vivian Lee |
|
| GFAP |
MP Biomedical |
0869110 |
| poly-L-lysine |
Merck |
P5899 |
| coated glass coverslips |
Stellar Scientific |
801007 |
| MEM medium |
Merck |
M0450 |
| MEM NEAA |
gibco/ThermoFisher Scientific |
11140050 |
| 10% FBS |
Merck |
F4135 |
| D-glycerophosphate |
Merck |
G9422 |
| dexamethasone |
Merck |
D4902 |
| L-ascorbic acid 2-phosphate |
Merck |
A8960 |
| 1% Pen-Strep |
gibco/ThermoFisher Scientific |
15070-063 |
| Paraformaldehyde (PFA) 16% Solution |
Electron Microscopy Sciences |
REF: 15710 |
| Alcian Blue |
Merck |
TMS-010 |
| 96-well round bottom ULA plates |
Greiner |
650185 |
| aMEM |
Merck |
M0450 |
| 10 % FBS |
Merck |
F4135 |
| TGF-b3 |
ThermoFisher |
100-36E-100UG |
| dexamethasone (Merck, D4902) |
Merck |
D4902 |
| L-Ascorbic acid |
Sigma-Aldrich |
A92902 |
| poly-L-lysine |
Merck |
P5899 |
| high glucose DMEM |
Merck |
D5671 |
| sodium pyruvate |
ThermoFisher |
11360070 |
| 10 % FBS |
Merck |
F4135 |
| TGF-b1 |
ThermoFisher |
100-21-100UG |
| PDGF-bb |
ThermoFisher |
100-14B-100UG |
| 1x PBS |
gibco/ThermoFisher Scientific |
14180-144 |
| B27 (50x) |
gibco/ThermoFisher Scientific |
17504-044 |
| L-glutamate |
Life Technologies |
25030149 |
| Glial cell-derived neurotrophic factor (rH-GDNF) |
STEMCELL Technologies |
78058.1 |
| Brain-derived neurotrophic factor (rH-BDNF) |
STEMCELL Technologies |
78005.1 |
| Nerve growth factor (rH-NGF) |
STEMCELL Technologies |
78092.1 |
| dibutyryl cyclic adenosine monophosphate (cAMP) |
TOCRIS-biotechne |
14A/226424 |
| retinoic acid (RA) |
MP Biomedicals, LLC |
190269 |
| 10% Knockout serum replacement (KSR) |
gibco/ThermoFisher Scientific |
10828010 |
| LDN-193189 |
MedChemExpress |
HY-12071 |
| A83-01 |
MedChemExpress |
HY-10432 |
| CHIR99021 |
Biogems |
2520691 |
| SB431542 |
Selleckchem |
S1067 |
| EDN3 |
Sigma-Aldrich |
E9137 |
| Human SCF |
Peprotech |
300-07-50UG |
| Essential 6 medium |
gibco/ThermoFisher Scientific |
A1516401 |
| TrypLE™ Express Enzyme (1X), no phenol red |
gibco/ThermoFisher Scientific |
12604013 |
| MCDB201 |
Millipore Sigma |
M6770-1L |
| 0.8% ITS+ (Insulin-Transferrin-Selenium (ITS-G) (100X)) |
gibco/ThermoFisher |
41400045 |
| SB431542 |
STEMCELL Technologies |
72234 |
| RO4929097 |
MedChemExpress |
HY-11102 |
| SU5402 |
Cal Biochem |
572630-500116 |
| Retinoic Acid |
Sigma-Aldrich |
R2625 |
| Bovine Albumin Fraction V |
MP Biomedicals |
810033 |
| recombinant human BMP4 |
R&D Systems/invitrogen |
314-BP/PHC9534 |
| PMA |
Sigma-Aldrich |
P1585 |
| Phosphate Buffer Saline (PBS) pH 7.4 (1X) |
gibco/ThermoFisher Scientific |
10010023 |
| Triton™X-100 |
The Dow Chemical Company |
1002614890 |
| SecureSeal Imaging Spacers |
Grace BIO-LABS |
654004 |
| ProLong Glass Antifade mounting media |
Invitrogen/ThermoFisher |
P36934 |
| Clear Nail Polish |
Electron Microscopy Sciences |
72180 |
| MatTek glass bottom dishes |
MATTEK, A BICO COMPANY |
P35G-1.5-20-C |
| 2-Mercaptoethanol |
BIO-RAD |
1610710 |
| NucleoSpin® RNA Isolation Kit |
Macherey-Nagel |
740955.50 |
| Multi Tissue Dissociation Kit 3 |
Miltenyi Biotec |
130-110-204 |
| 70 μm MACS SmartStrainer |
Miltenyi Biotec |
130-098-462 |
| Chromium Single Cell 3′ Library & Gel Bead Kit v3 |
10X Genomics |
|
| Illumina NextSeq 500 Platform |
|
|
