Fig 8. Annual change during the 65-year period analyzed (1956-2021) of mean environmental temperature and Wb-bs index in two selected meteorological locations (Gobernador Gregores and San Julian) placed between latitudes 48° and 50° S, where the geographical spread of the fascioliasis transmission area has occurred.

In each panel, picture (1) shows the mean environmental temperature annual trend, represented by the observed values (grey columns), the observed trend obtained from the Seasonal-Trend decomposition procedure (black line), and the regression line (red-slashed line) (the slashed grey line depicts the 10 °C threshold required for the life cycle of Fasciola hepatica to progress). Picture (2) shows the trend of the annual number of days with mean temperature above 10 °C, represented by the observed values (black dots), and the regression line obtained from generalized linear models (red-slashed line). Picture (3) represents the mean monthly change of the Wb-bs index by intervals of 10 years (the slashed grey line depicts the value of 600, which indicates risk of transmission. Picture (4) shows the Wb-bs index annual trend, represented by the observed values (grey columns), the observed trend obtained from the Seasonal-Trend decomposition procedure (black line), and the regression line (red-slashed line) (the slashed grey line depicts the value of 600, which indicates risk of transmission). For an improved readability, it is suggested to visualize this figure in its digital version.